Hi,
No I don't use super resolution.
The header from gain is:
RO image file on unit 1 : FoilHole_6873894_Data_6874760_6874762_20190807_1659_gain.tiff Size= 68068 K
This is a TIFF file.
Number of columns, rows, sections ..... 11520 8184 1
Map mode .............................. 0 (byte)
Start cols, rows, sects, grid x,y,z ... 0 0 0 11520 8184 1
Pixel spacing (Angstroms).............. 1.000 1.000 1.000
Cell angles ........................... 90.000 90.000 90.000
Fast, medium, slow axes ............... X Y Z
Origin on x,y,z ....................... 0.000 0.000 0.000
Minimum density ....................... 0.0000
Maximum density ....................... 255.00
Mean density .......................... 127.50
tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0
Space group,# extra bytes,idtype,lens . 0 0 0 0
0 Titles :
The header from the movie is:
RO image file on unit 1 : FoilHole_6873900_Data_6874760_6874762_20190807_1703_fractions.tiff Size= 158758 K
This is a TIFF file.
Number of columns, rows, sections ..... 5760 4092 37
Map mode .............................. 0 (byte)
Start cols, rows, sects, grid x,y,z ... 0 0 0 5760 4092 37
Pixel spacing (Angstroms).............. 1.000 1.000 1.000
Cell angles ........................... 90.000 90.000 90.000
Fast, medium, slow axes ............... X Y Z
Origin on x,y,z ....................... 0.000 0.000 0.000
Minimum density ....................... 0.0000
Maximum density ....................... 255.00
Mean density .......................... 127.50
tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0
Space group,# extra bytes,idtype,lens . 0 0 0 0
0 Titles :
________________________________
From: Takanori Nakane <[log in to unmask]>
Sent: Thursday, August 8, 2019 2:51:43 PM
To: Yehuda Halfon <[log in to unmask]>
Cc: [log in to unmask] <[log in to unmask]>
Subject: Re: [ccpem] The new embedded K3 in EPU gain reference file
Hi,
I need sample files to investigate this issue.
Are you using super-resolution?
Can you run IMOD's "header" command on both the gain reference
and the movie from EPU?
Best regards,
Takanori Nakane
> Hi all,
>
> We just got an update for EPU that let us work with K3.
>
> In it we can now save files in LZW tif without the gain. However the gain
> file is also in tiff and in its size (in pixels) is bigger but the ratio
> is the same. Relion complains about it and won't do mcorr. So I resized it
> to the K3 size, converted it to mrc using tif2mrc, and now everything
> works fine.
>
>
> I have 2 questions:
>
> 1) Is the resizing it will make the mcorr run worst?
>
> 2) Anyone knows if I can save the gain file in mrc in the correct size?
> (like from GMS?)
>
>
> Thanks,
>
>
> Yehuda Halfon
>
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