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Hi, These commands should work. > Also the size becomes different from the particle size (400 X 400) > and the output spectrum is with the size (210 X 400) Are you sure it was 210? It should be 201 x 400, not 210 x 400. Because the input images are real numbers, the reciprocal space has Hermitian symmetry (= Friedel's law in crystallography). So we write only half of the reciprocal space. Best regards, Takanori Nakane > Hi RELION developers, > I have noticed in relion_image_handler module, there are three option > --avg_ampl --avg_ampl2 --avg_ampl2_ali and from the description, it seems > being able to generate average power spectrum. However, when I quickly > tried with my particle star files with any one of these options specified, > the output was not anything meaningful. Also the size becomes different > from the particle size (400 X 400) and the output spectrum is with the > size (210 X 400), which seems confusing. I am wondering if this is a fully > developed function or did I do something wrong? > Best, > Yangqi > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1