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ALS 822. I tried as low as 0.2 sec exposure but it didn't seem to help much.


Kimberly Stanek
Postdoctoral Researcher, Goulding Lab
Co-chair, UCI Postdoctoral Association
University of California, Irvine
Natural Science I, Room 2302
(949) 824-0014
From: James Holton <[log in to unmask]>
Sent: Thursday, August 29, 2019 10:50 AM
To: Kimberly Stanek <[log in to unmask]>; [log in to unmask] <[log in to unmask]>
Subject: Re: [ccp4bb] Semet derivative dying almost immediately in beam
 
What exposure time are you using?  And which beamline? 

-James Holton
MAD Scientist

On 8/29/2019 10:26 AM, Kimberly Stanek wrote:
Hi folks,

We have a protein that we have been trying to solve the structure of for a while now but so far haven't been able to get any diffraction better than ~4.5A. I was able to collect a full 360 degrees of data and index, but MR is failing so we have turned to de novo phasing.

Recently we prepared crystals of the Semet derivative under the same condition. While these crystals diffracted to about the same resolution, I found they were dying after just one or two snaps, even with increased beam attenuation and decreased exposure time. I am wondering if anyone has experienced anything like this before and had any suggestions on what to do about it.

Thank you,



Kimberly Stanek
Postdoctoral Researcher, Goulding Lab
Co-chair, UCI Postdoctoral Association
University of California, Irvine
Natural Science I, Room 2302
(949) 824-0014

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