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Hi Ramesh,
   You can create as many masks as you want, as long as you have a prior belief for how you will interpret results for all these masks ( e.g. do you have a hypothesis for all masks as a whole or a number of separate unrelated hypotheses? ) .

Kind Regards
Matthew
--------------------------------
Dr Matthew Webster
FMRIB Centre
John Radcliffe Hospital
University of Oxford

On 18 Jun 2019, at 22:12, Ramesh Babu <[log in to unmask]<mailto:[log in to unmask]>> wrote:

Dear Expert,
Sorry for reposting this question.

I am doing VBM analysis and I need to create several masks. I have a few questions related to this.
Are there any limitations to creating masks? If there are more than five masks, for example in the frontal region, should I create a union of mask or can create a separate mask? Please let me know.

Thanks in advance
RB

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