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This is my current record of Cs values on TFS systems for bio, maybe TFS people on this list can confirm these:

Microscope			pole type		Cs [mm]

Tecnai 10				High-Contrast	4.3
	
Tecnai 12  			Bio-Twin		6.3

Tecnai 12				Twin			2.26
Tecnai (F)20			Twin			2.26
Tecnai (F)30/Polara		Twin			2.26
	
Glacios				C-twin		2.7
Tecnai Arctica			C-twin		2.7
Talos Arctica			C-twin		2.7
Titan Krios & Halo		C-twin		2.7

Best,

Wim Hagen
EMBL Heidelberg


> On 13. Jun 2019, at 13:59, Grassucci, Robert <[log in to unmask]> wrote:
> 
> Our Polara has a Cs of 2.26 mm.  This was actually measured before it was installed but most twin lenses are said by Philips/FEI/Thermofisher  to have a Cs of 2.0 mm.
> Bob
>  
> From: Collaborative Computational Project in Electron cryo-Microscopy [mailto:[log in to unmask] <mailto:[log in to unmask]>] On Behalf Of sunyeping
> Sent: Wednesday, June 12, 2019 4:07 AM
> To: [log in to unmask] <mailto:[log in to unmask]>
> Subject: [ccpem] what is the spherical aberration of TF30 Polara electron microscope (FEI)
>  
> Dear everyone, 
>  
> I am trying to process microscophs data collected from TF30 Polara electron microscope with relion. The data is downloaded from EMPIAR. In the "CTF estimation" work type, the I/O tag interface require to input the  spherical aberration. Do you know what is the spherical aberration value of FEI TF30 Polara electron microscope?
>  
> Best regards
>  
> Yeping
>  
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