This is my current record of Cs values on TFS systems for bio, maybe TFS people on this list can confirm these: Microscope pole type Cs [mm] Tecnai 10 High-Contrast 4.3 Tecnai 12 Bio-Twin 6.3 Tecnai 12 Twin 2.26 Tecnai (F)20 Twin 2.26 Tecnai (F)30/Polara Twin 2.26 Glacios C-twin 2.7 Tecnai Arctica C-twin 2.7 Talos Arctica C-twin 2.7 Titan Krios & Halo C-twin 2.7 Best, Wim Hagen EMBL Heidelberg > On 13. Jun 2019, at 13:59, Grassucci, Robert <[log in to unmask]> wrote: > > Our Polara has a Cs of 2.26 mm. This was actually measured before it was installed but most twin lenses are said by Philips/FEI/Thermofisher to have a Cs of 2.0 mm. > Bob > > From: Collaborative Computational Project in Electron cryo-Microscopy [mailto:[log in to unmask] <mailto:[log in to unmask]>] On Behalf Of sunyeping > Sent: Wednesday, June 12, 2019 4:07 AM > To: [log in to unmask] <mailto:[log in to unmask]> > Subject: [ccpem] what is the spherical aberration of TF30 Polara electron microscope (FEI) > > Dear everyone, > > I am trying to process microscophs data collected from TF30 Polara electron microscope with relion. The data is downloaded from EMPIAR. In the "CTF estimation" work type, the I/O tag interface require to input the spherical aberration. Do you know what is the spherical aberration value of FEI TF30 Polara electron microscope? > > Best regards > > Yeping > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCPEM-26A-3D1&d=DwMGaQ&c=G2MiLlal7SXE3PeSnG8W6_JBU6FcdVjSsBSbw6gcR0U&r=DHQ5sxLdEeh7GliHapLyXDQKaXurrg9EcaYzNZr3hks&m=HhB7KwpDRx-y00K5v_CsXbMYPo9AgkohhqIRJnQ6ayk&s=SX1mZsCL6ANpU4jrqQTVXlfIzVDRTgDwPq5MqvBrtdQ&e=> > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1> ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1