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Hi, No. It says "AT LEAST approximately 100 particles per class". It is completely fine to use more particles par class. I don't use more than 200 classes, even if I have 1 million particles. Best regards, Takanori Nakane > Dear everyone, > > In the section 1.7 (Making templates for auto-picking) of the relion30 > tutorial, about "the number of classes" on the "optimisation" tab, the > tutorial states that "we like to use on average at least approximately 100 > particles per class". The data I am working on have 1200 micrographs and > about 1.6 million particles have been extracted. If there are 100 > particles in one class, then there should be 1,600 classes. So should I > set "the number of classes" to 1600? > > Best regards > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1