Hi, > I am unable to use the GPU with this option. This is expected. It doesn't use GPU. > The log file indicates that: Written aligned but non-dose weighted sum > to MotionCorr/job019/Micrographs/*_noDW.mrc > But there is no *noDW.mrc file in the directory. > only *.mrc files are there. Oops, the message is incorrect. When you specify 'dose weighting: no', non-dose weighted sums are called *.mrc, not *_noDW.mrc. The files are fine; just proceed your processing. By the way, I always apply dose weighting. Indeed, CTF estimation might work bit better on non-dose weighted images but RELION 3.0 has CtfRefine. Writing non-dose weighted images is waste of disk in most cases. Best regards, Takanori Nakane ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1