Hi, For per-micrograph defocus, astigmatism and Cs, "--premultiply_ctf" option in relion_preprocess should work. More complicated cases (beam tilt, per-particle defocus) are not supported in 3.0.x. This issue will be resolved in 3.1, which is not yet available. > the CTF of the particles are not corrected, right? You are right. CTF correction is done on-the-fly during refinement and reconstruction. Best regards, Takanori Nakane On 2019/04/29 20:03, Yangqi Gu wrote: > Dear all, > I am wondering if anyone know how to generate CTF corrected particles in RELION? I assume after extraction, the CTF of the particles are not corrected, right? > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1