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Mohinder, You could also try HPLC where you should get better results due to more theoretical plates. One option would from Phenomenex. 10E6A Phase Information ------------------------------ - Molecular Weight Range: 60 K - 10,000 K https://www.phenomenex.com/Products/HPLCDetail/phenogel *David L. Blum, Ph.D.* *Bioexpression and Fermentation Facility | Director* *Master of Biomanufacturing and Bioprocessing | Director* *120 Green Street | Life Sciences Bldg rm A414A | Athens, GA 30602 * *706-542-1035* <706-542-1035>* | **[log in to unmask]* <[log in to unmask]>* | * *bff.uga.edu* <http://bff.uga.edu/>* | **biomanufacturing.uga.edu* <http://biomanufacturing.uga.edu/> On Wed, Apr 17, 2019 at 11:11 AM Mohinder Pal <[log in to unmask]> wrote: > Dear all, > > I would like to gel filter a multi protein complex (1.4MDa) as the final > purification step. I have tried tandem Superose 6 columns and this complex > elutes close to void volume as it is a very elongated molecule. > > > I was wondering if someone could suggest different analytical columns for > better resolution as I plan to add more components to this existing > complex. > > Best wishes, > > Mohinder Pal > > ---------------------------------------------- > "Whatever you’re meant to do, do it now. The conditions are > always impossible.” > Doris Lessing > ---------------------------------------------- > > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1