Dear Pranav Shah

Our camera normalisation program should be able to correct your "screwey gain reference" treated data set... It improves even on "correctly" gain corrected data sets!

The condition is that the dataset should be large enough (> ~1000 movie frames). Moreover the "screwey gain reference", that you corrupted your data with, should not contain anomalities, like both negative and positive values, or undefinined/overflow floating point values. That being the case you should able to salvage the data with our program.

Cheers

Marin

For details see: https://www.nature.com/articles/srep10317

On Thu, Mar 14, 2019 at 5:27 AM Pranav Shah <[log in to unmask]> wrote:

Dear Community,

Due to an inadvertent mistake I ended up collecting a dataset
normalised with a screwey gain ref and I was wondering if there was an
in silico way to fix this mistake. Has anyone had a similar problem
and managed to save their precious data?

Looking forward to your suggestions.
Cheers,
Pranav
--
Pranav Shah
Postdoctoral Research Fellow.

Hogle Lab
Department of Biological Chemistry and Molecular Pharmacology,
Building C2 - First Floor
240 Longwood Avenue
Boston, MA 02115
(617) 432-4360 (fax)
(617) 432-3839 (lab)

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