Hi Yi Zhang

As Sharan suggests microseeding may be the key to solving your problem. ‘Random’ microseeding or MMS, where seed stock is added to random screens may be helpful. It often gives new hits, crystals that diffract better and may allow you to control the number of crystals per drop (if you dilute the seed stock).

A very good reference for this is:

or search online for MMS microseeding or rMMS microseeding.

Hope it works for you

Stefan

Stefan Kolek

Douglas Instruments Ltd, Hungerford, Berkshire, RG17 7HD, UK

Email: [log in to unmask] 

Gmail: [log in to unmask]

Tel:  +44 (0) 1488 649090 US toll free: 1-877-225-2034

Web: www.douglas.co.uk



On Fri, 22 Mar 2019 at 11:57, Sharan Karade <[log in to unmask]> wrote:
Dear Jun 

Did you tried seeding the old crystals. Just crush them and make different dilutions with same buffer. Hope, it will work out for you.

Regards
Sharan



Fri, Mar 22, 2019, 6:55 AM 马俊 <[log in to unmask]> wrote:
Dear all,
   A single positive stranded RNA virus RDRP grows crystals, monomers, precipitants (PEG8000)

with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution. Precipitant concentration increased, many crystal nucleus, crystal is very small.  Try to optimize the temperature, pH, different protein concentration, dehydration, additives, detergent, sit and hanging drop, but were not improved much.  Pease provide some advice methods.

Thanks

Jun Ma



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