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Hi Aleix,
Can you describe how you did the fluorescence scan, or share the plot itself? I am asking this because you say you could discard Fe, Zn and As/Se but you don't say anything about Cu which is in between these elements.
If you tried but could not discard Cu, then it could be your "Culprit".
Best,
Nukri

On Fri, Mar 15, 2019 at 4:17 PM Aleix Tarrés Solé <[log in to unmask]> wrote:
Dear colleagues:
Hereby, I would like to expose a problem I encountered during the data processing of one of my crystals. Recently I collected a full dataset of a native protein-DNA crystal at an energy of 12667KeV. To my surprise, after processing with XDS I can see that I have significant values of anomalous correlation up to 5 Angstrom resolution. Using the fluorescence scan on the beam, I could discard the presence of the following heavy atoms that could have ended up in my drop due to cross-contamination; Se, As, Fe, Zn and Pr. So far, my attempts to use this dataset for phasing have been sadly unsuccessful. I would like to know if somebody has experience in this kind of problem and can gently help with some advices. 

Thank you all in advance

Aleix


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