Dear Almu,

We have a discussion on the Phaser Wiki about some of the possibilities for tNCS, what you should look for, and what Phaser can handle: http://www.phaser.cimr.cam.ac.uk/index.php/Molecular_Replacement#Translational_Non-crystallographic_Symmetry.  As it explains, if you have one major peak in the native Patterson then everything will be pretty automatic, and it can even be straightforward if you have several peaks corresponding to multiples of the same underlying translation.  If you have some specific questions about your problem after you’ve read that and run Phaser on your problem, then let us know!

Best wishes,

Randy

-----
Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research    Tel: +44 1223 336500
Wellcome Trust/MRC Building                         Fax: +44 1223 336827
Hills Road                                                            E-mail: [log in to unmask]
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> On 14 Mar 2019, at 05:07, Almudena Ponce Salvatierra <[log in to unmask]> wrote:
>
> Dear all,
>
> I have a dataset with strong TNCS and I would like to know if there are a "series of steps" that I could follow in order to find out whether I can solve my structure or not.
>
> I see on the Phaser wiki that structure determination in the presence of TNCS is not fully automated. which steps are those in which my intervention should be needed and what do I look for?
>
> It is the first time that I deal with this problem and I'd be immensely thankful if I could get some hints from those of you who have encountered it in the past or understand where in particular I should keep and eye on :)
>
> All the best,
>
> cheers,
>
> Almu
>
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