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We used to use 1% solution of dimethyl dichloro silane in toluene to siliconize glass coverslips. Soak them for 30 min, rinse well with deioinized water, and oven/air dry.
Uma.

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Uma Gabale, PhD
Research Associate
Molecular and Cellular Biochemistry
Indiana University Bloomington


On Thursday, January 31, 2019, 3:17:37 AM EST, Rajnandani Kashyap <[log in to unmask]> wrote:


Dear All
I am a PhD student who requires lots of coverslips (!!) for setting up hanging drop crystallization. The company sells it for a huge amount. Also there is a wide monetary difference between a normal siliconized coverslip and a 22mm siliconized circle coverslips. We tried to search for an alternative companies but couldn't get any one who sells coverslips with the same dimensions (0.19-0.22mm glass thickness and 22 mm glass diameter). Is there any alternative company (distribution in India) from where we can buy them for a reasonable price?
Thanks in advance for sparing your valuable time and efforts.

Regards
Rajnandani Kashyap
India


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