Dear ccpem and cryoEM experts,

I have a naive question about CTF refinement in Relion 3.0 and I am never fully convinced. For squeezing the high resolution, does it make sense to perform beam tilt estimation in a data set that was collected shooting just one acquisition per hole? 

Thank you very much.

Best,

Hugo

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Hugo Muñoz Hernández 
Postdoctoral Fellow, Maier lab
Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH–4056 Basel
Phone : +41 61 207 21 03 
[log in to unmask] www.biozentrum.unibas.ch

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