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Hi Ashu, Are you imposing symmetry? P > Il giorno 4 gen 2019, alle ore 18:31, Ashu Sharma <[log in to unmask]> ha scritto: > > Dear All, > > On a single particle cryo-EM data refined in relion 2, I wanted to do Bayesian polishing. And therefore after MotionCorr2 etc. in Relion 3 pipe line, I did a 3D refinement on exactly the same set of particles which I used in Relion 2 for auto-refine. > Refinement converges to the same resolution as Relion 2, map looks the same. But in Relion 3 post-processing, none of the FSC curves except the one for phase-randomized masked maps ever touches zero. They plateau around 0.02-0.03. I tried playing with wider/softer masks but that doesn't help. > > What could be going wrong? > > Thanks a lot ! > Ashu > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1