Dear Mohinder,
Fortunately, you don’t have to manually pick again. Relion 3.0 can import the coordinates from previous versions.
Have a good day.
Best,
Hugo
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Hugo Muñoz Hernández
Postdoctoral Fellow, Maier lab
Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH–4056 Basel
Phone : +41 61 207 21 03
[log in to unmask] www.biozentrum.unibas.ch
On 23 Jan 2019, at 15:44, Mohinder Pal <[log in to unmask]> wrote:
Dear all,
I have refined the structure using relion 2.1 and later on used relion 3.0 for multi body refinement.
Now I would like to do particle polishing.
I have read a previous thread advising to do motion corrections again in relion 3.0.
Does this mean that I have to manually pick particles again using new Motion corrected micrographs (using relion 3.0) to solve the structure from scratch?
Best wishes,
Mohinder Pal
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"Whatever you’re meant to do, do it now. The conditions are always impossible.”
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