Dear Mohinder,

Fortunately, you don’t have to manually pick again. Relion 3.0 can import the coordinates from previous versions.

Have a good day.

Best,

Hugo

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Hugo Muñoz Hernández 
Postdoctoral Fellow, Maier lab
Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH–4056 Basel
Phone : +41 61 207 21 03 
[log in to unmask] www.biozentrum.unibas.ch

On 23 Jan 2019, at 15:44, Mohinder Pal <[log in to unmask]> wrote:

Dear all,

I have refined the structure using relion 2.1 and later on used relion 3.0 for multi body refinement. 

Now I would like to do particle polishing. 

I have read a previous thread advising to do motion corrections again in relion 3.0.

Does this mean that I have to manually pick particles again using new Motion corrected micrographs (using relion 3.0)  to solve the structure from scratch?

Best wishes,

Mohinder Pal

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"Whatever you’re meant to do, do it now. The conditions are always impossible.” 
Doris Lessing
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