I have a naive question about CTF refinement in Relion 3.0 and I am never fully convinced. For squeezing the high resolution, does it make sense to perform beam tilt estimation in a data set that was collected shooting just one acquisition per
hole?
Thank you very much.
Best,
Hugo
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Hugo Muñoz Hernández
Postdoctoral Fellow, Maier lab
Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH–4056 Basel
Phone : +41 61 207 21 03 [log in to unmask]www.biozentrum.unibas.ch