Dear ccpem and cryoEM experts, I have a naive question about CTF refinement in Relion 3.0 and I am never fully convinced. For squeezing the high resolution, does it make sense to perform beam tilt estimation in a data set that was collected shooting just one acquisition per hole? Thank you very much. Best, Hugo —————————————————— Hugo Muñoz Hernández Postdoctoral Fellow, Maier lab Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH–4056 Basel Phone : +41 61 207 21 03 [log in to unmask]<mailto:[log in to unmask]> www.biozentrum.unibas.ch<http://www.biozentrum.unibas.ch> ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1