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Hi Mohinder, No. You only need to re-run the Motion-correction job in relion-3.0, and use the output STAR file as input into Bayesian polishing, together with the 3D refinement you already did (in 2.1 or 3.0 that doesn't matter). HTH, Sjors > Dear all, > > I have refined the structure using relion 2.1 and later on used relion 3.0 > for multi body refinement. > > Now I would like to do particle polishing. > > I have read a previous thread advising to do motion corrections again in > relion 3.0. > > Does this mean that I have to manually pick particles again using new > Motion corrected micrographs (using relion 3.0) to solve the structure > from scratch? > > Best wishes, > > Mohinder Pal > > ---------------------------------------------- > "Whatever you’re meant to do, do it now. The conditions are always > impossible.” > Doris Lessing > ---------------------------------------------- > > > > > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > -- Sjors Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH, U.K. tel: +44 (0)1223 267061 http://www2.mrc-lmb.cam.ac.uk/groups/scheres ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1