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Hi Takanori, Indeed there a duplicate entries ! I don't know when and how did that happen? Thanks so much ! Ashu On Fri, Jan 4, 2019 at 12:39 PM Takanori Nakane <[log in to unmask]> wrote: > Hi, > > Did you make sure there are no duplicated particles? > > Best regards, > > Takanori Nakane > > > Hi Ashu, > > > > Are you imposing symmetry? > > > > P > > > >> Il giorno 4 gen 2019, alle ore 18:31, Ashu Sharma > >> <[log in to unmask]> ha scritto: > >> > >> Dear All, > >> > >> On a single particle cryo-EM data refined in relion 2, I wanted to do > >> Bayesian polishing. And therefore after MotionCorr2 etc. in Relion 3 > >> pipe line, I did a 3D refinement on exactly the same set of particles > >> which I used in Relion 2 for auto-refine. > >> Refinement converges to the same resolution as Relion 2, map looks the > >> same. But in Relion 3 post-processing, none of the FSC curves except the > >> one for phase-randomized masked maps ever touches zero. They plateau > >> around 0.02-0.03. I tried playing with wider/softer masks but that > >> doesn't help. > >> > >> What could be going wrong? > >> > >> Thanks a lot ! > >> Ashu > >> > >> To unsubscribe from the CCPEM list, click the following link: > >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > > > > ######################################################################## > > > > To unsubscribe from the CCPEM list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > > > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1