Hi Martyn

Thanks for your reply and the link.

Its helpful…

 

Regards

Monika

 

 

Use CCP-EM!  Which has your favourite CCP4 programs adapted for cryoEM, plus some others. See www.ccpem.ac.uk

 

There is a hierarchy from rigid body fitting (of your xtal monomer), to flexible fitting (to account for adjustments in the new form), to de novo building and refinement. What you can do will depend on the EM resolution (local as well as global).

 

Look out for our annual Icknield workshops that provide training on model fitting and building.

 

HTH

Martyn

 

 

Hi there

 

Please can I get some suggestion on how to build a pdb into a cryoEM map.

To be more clear I have a crystal structure solved as dimer in asymmetric unit and I am trying to model it as a trimer form (biologically relevant) using cryo-EM map of a very similar homologue protein as a template.

I thank in advance for your help.

Thanks

Regards

 

Monika

School of Life Sciences

University of Nottingham

UK

 

 

 

 

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