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Hi Martyn Thanks for your reply and the link. Its helpful... Regards Monika From: Martyn Winn - UKRI STFC <[log in to unmask]> Sent: 04 December 2018 11:44 To: Monika Pathak <[log in to unmask]>; [log in to unmask] Subject: RE: fit pdb on cryo-EM map Use CCP-EM! Which has your favourite CCP4 programs adapted for cryoEM, plus some others. See www.ccpem.ac.uk<http://www.ccpem.ac.uk> There is a hierarchy from rigid body fitting (of your xtal monomer), to flexible fitting (to account for adjustments in the new form), to de novo building and refinement. What you can do will depend on the EM resolution (local as well as global). Look out for our annual Icknield workshops that provide training on model fitting and building. HTH Martyn From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Monika Pathak Sent: 04 December 2018 11:04 To: ccp4bb Subject: [ccp4bb] fit pdb on cryo-EM map Hi there Please can I get some suggestion on how to build a pdb into a cryoEM map. To be more clear I have a crystal structure solved as dimer in asymmetric unit and I am trying to model it as a trimer form (biologically relevant) using cryo-EM map of a very similar homologue protein as a template. I thank in advance for your help. Thanks Regards Monika School of Life Sciences University of Nottingham UK This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please contact the sender and delete the email and attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. Email communications with the University of Nottingham may be monitored where permitted by law. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please contact the sender and delete the email and attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. Email communications with the University of Nottingham may be monitored where permitted by law. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1