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Hello All, I am trying to refine some protein structure and right now my structure's R-free is not coming down from 37% even the R-work is lowering in each round of refinements. When phasing done, it created 8 chains for dimer protein (~750 aa's per unit). So I am using NCS for refinement in each cycle. My question is that how to choose the best chains to refine in coot before I do the SSM superpose, Merge and then run the refinement. I am also trying to try auto-build if R-values don't come down. Any suggestions or education is welcomed. Thanks and cheers. Chetan Arya Instem, Bangalore India 560065 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1