Hello,
I am interested in aligning all my particles, and as I have been looking through the Relion documentation, It seems that through the 2D classification step, the particles can be aligned. However, It takes time to create all the 2D classes and I was wondering if there was a less computationally expensive way of aligning the particles or a way to just parse part of the relion_refine method to only align all the particles without having to create the 2D class averages.
Lieza M Chan
Postbaccalaureate Research Fellow
National Institute of Diabetes and Digestive and Kidney Diseases
National Institutes of Health
8 Center Dr, Bethesda MD 20892
email: [log in to unmask]
phone: 301.827.3604
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