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Dear Catarina, You may recopy the last volume and rename it as the 0 volume to become the first volume. Then you may remove this file after realignment procedure the data. Also, you should remove its values from the realignment file. Best Regards Rouhi *Institut für Neurowissenschaften und Medizin (INM-3)* Forschungszentrum Jülich Leo-Brandt-Straße 52425 Jülich, GermanyTel: +49 2461 61-4686 On Thu, Oct 4, 2018 at 11:11 AM Catarina Rua <[log in to unmask]> wrote: > Dear SPMers, > > I would like to use SPM's realign function to realign an fmri time series > to the last acquired volume. Since the fags we can select are either to > realign to the mean of the time series or the first image of the time > series, can I simply flip the data having the last volume acquired as > volume 1, then realign using: flags.rtm = 0; and then flip back the > time series? > Is this procedure isn't very neat, would you advice some other method? > Thank you very much in advance. > > Best wishes, > Cat >