Hi,
It's probably possible to script what you want, but since importing your CARA data again and associating the assignments with the correct sequence should be easy, that's probably the better way to go.
The lua script
http://wiki.cara.nmr.ch/WriteSparkyPeakList.lua
should allow you to export your assigned peak list(s) from CARA and these should be straightforward to import into CCPN analysis.
Dr. Brian O. Smith --------------------------- Brian Smith at glasgow ac uk
Institute of Molecular, Cell and Systems Biology & School of Life Sciences,
College of Medical, Veterinary & Life Sciences,
Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
Tel: 0141 330 5167/6459/3089
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The University of Glasgow, charity number SC004401
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From: CcpNmr software mailing list [[log in to unmask]] on behalf of Muhd Mohd Kipli [[log in to unmask]]
Sent: 29 October 2018 20:04
To: [log in to unmask]
Subject: Started with the wrong amino acid sequence at the beginning of a project - assignment requires shifting
Hi Everyone,
I have been using CARA for assignment and been transferring my assignment to Ccpnmr analysis version 2.2.4. to follow chemical shift changes for titration experiment.
Problem:-
The initial sequence that I had put into analysis project is wrong - it includes 3 amino acids from cloning artefacts that had been removed. The sequence that I used to do assignment in CARA excludes these 3 amino acid artefacts and I have been using these assignments when transferring these assignments into analysis.
So all the assigned amino acids are shifted by 3 amino acids and it’s all wrong.
I was wondering if there is any way for me to fix this without having to create a new project, inserting the correct sequence (without the 3 amino acids artefacts) and transferring all the assignments into every spectrum for every titration that I did.
Does anyone have any experience in such an issue? This would save a lot of time by avoiding creating a new project. Any help would be greatly appreciated.
Note:-
I tried making a new chain, chain B with the correct sequence and transferring the assignment from the original chain to chain B. But this does not shift the assignment three amino acids along the sequence.
Many Thanks,
Faiz
DPhil (PhD) Student in Biochemistry,
Schnell Group,
University of Oxford.
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