Hi Thomas and Joe, 2D classification works after splitting the data into two halves. Hopefully this issue will be resolved in future releases. Best > On Oct 26, 2018, at 5:14 AM, Joseph Atherton <[log in to unmask]> wrote: > > Yes, splitting the data in half worked nicely for me too when I had this error. > > Cheers, > Joe > >> On 25/10/2018, 20:27, Yu Xinchao wrote: >> Thanks Thomas, I do have a very large data set (9000+ movies) and that might be the reason. I will try split the data in half and rerun it. >> Best >> Xinchao >> >> Sent from my iPhone >> >>> On Oct 25, 2018, at 12:22 PM, Thomas Bausewein <[log in to unmask]> wrote: >>> >>> Hi Xinchao, >>> >>> im my case I had a really large dataset and the set2Dslice error vanished when I split the dataset in half (using the Relion GUI) and classified the smaller datasets individually. >>> I did not remove any particles, neither from the folders nor from the particle.star files. >>> >>> You should also do relion_image_handler --stats --i Extract/Job006/particles.star in your project folder and not in the Extract/job006 folder, since it reads the path that is written in the particles.star file. >>> >>> Thomas >>> >>> >>>> On 25 Oct 2018, at 20:59, Xinchao Yu <[log in to unmask]> wrote: >>>> >>>> Hi All, I got the the same error message when I tried to do 2D classfication: Backprojector::set2DSlice%%: Dimension of the data array should be 2 or 3 >>>> I was able do MotionCor2, Gctf, Autopicking and particle extraction without any error messages. >>>> I checked the files with relion_image_handler --stats --i particles.star and it's always the first file that give the following message: >>>> ERROR: >>>> Cannot read file Extract/Job006/Micrographs/NP146-g1_06973.mrcs It does not exist. >>>> I checked the folder and the file does exist and when I deleted the file from the .star files I would get same error message for the next file. >>>> Does anyone have a solution to this? >>>> Thanks, >>>> Xinchao Yu >>>> >>>> ######################################################################## >>>> >>>> To unsubscribe from the CCPEM list, click the following link: >>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 >> ######################################################################## >> >> To unsubscribe from the CCPEM list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1