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Dear Relion users and developers,



I have two questions regarding motion correction with relion/3.0-beta-sm52



  1. I have been running motion correction with relion's own implementation but it skipped some of the movies and reported the following error message:


                      "too few valid local trajectories to fit local motion model"

           

           Does it indicate the bad movie quality? Or is it caused by wrong settings?


           Here is the printed command:


`which relion_run_motioncorr` --i Import/job001/movies.star --o MotionCorr/job018/ --first_frame_sum 1 --last_frame_sum 0 --use_own  --j 1 --bin_factor 1 --bfactor 150 --angpix 0.9 --voltage 300 --dose_per_frame 1.2 --preexposure 0 --patch_x 5 --patch_y 5 --gainref micrographs/Movies/Gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting




     2. When I run motioncor2 with relion's GUI, some of the movies were summed and corrected into totally black images. The other movies are fine. 


          Here are my settings for motioncor2:


           `which relion_run_motioncorr` --i Import/job001/movies.star --o MotionCorr/job018/ --first_frame_sum 1 --last_frame_sum 0 --use_motioncor2  --motioncor2_exe /apps/motioncor2/1.1.0/motioncor2 --gpu "2" --bin_factor 1 --bfactor 150 --angpix 0.9 --voltage 300 --dose_per_frame 1.2 --preexposure 0 --patch_x 5 --patch_y 5 --gainref micrographs/Movies/Gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting  


Has anyone also faced these problems before? And how did you work around them? Any help would be appreciated. 


Best,
Chen




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