Dear Relion users and developers,
I have two questions regarding motion correction with relion/3.0-beta-sm52.
"too few valid local trajectories to fit local motion model"
Does it indicate the bad movie quality? Or is it caused by wrong settings?
Here is the printed command:
`which relion_run_motioncorr` --i Import/job001/movies.star --o MotionCorr/job018/ --first_frame_sum 1 --last_frame_sum 0 --use_own --j 1 --bin_factor
1 --bfactor 150 --angpix 0.9 --voltage 300 --dose_per_frame 1.2 --preexposure 0 --patch_x 5 --patch_y 5 --gainref micrographs/Movies/Gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting
2. When I run motioncor2 with relion's GUI, some of the movies were summed and corrected into totally black images. The other movies are fine.
Here are my settings for motioncor2:
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