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Hi everyone! An apology in advance for the newbie question, I'm new to CryoEM and I'm trying to install RELION on a fresh installed Ubuntu 16.04 system, however after following the instructions in the README.md file of the relion folder (relion-3.0_beta) downloaded from ( https://bitbucket.org/scheres/relion-3.0_beta.git), and after installing the X11 library, I manage to follow the whole installation as indicated, however when I try to launch "relion", I get the message: command not found Would you think it's due to my version of Ubuntu? I could try ubuntu 14 or 17 as I have seen in some pages it may work, I attached the output of the terminal during the whole installation process, excuse me that is in spanish (as comment I had installed git and ran git clone in advance to the log file to obtain de README.md file). Any help or comments would be really appreciated, thank you very much for your time, wish you all an excellent and a productive week, see you soon! El sáb., 20 oct. 2018 a las 12:48, Sjors Scheres (<[log in to unmask]>) escribió: > Hi Oli, > In the extraction job type, you can now provide a 3D centre coordinates. > This will be projected onto the centre of the 2D particles. Is that what > you need? > Hth, > Sjors > > > > Sent from my phone. > > -------- Original message -------- > From: Oliver Clarke <[log in to unmask]> > Date: 12/10/2018 19:52 (GMT+00:00) > To: [log in to unmask] > Subject: [ccpem] relion_flex_analyse recentering without multi-body? > > Hi, > > In the RELION multi-body paper, it is stated that: > > “...the relion_flex_analyse program can output subtracted images according > to the iteratively refined relative orientations from a multi-body > refinement. The resulting subtracted particle images can then be used in > subsequent focused classifications and/or refinements. An additional > advantage of this program over previously existing subtraction tools in > relion is the centering at the projected centre-of-mass of the subtracted > particle images, which allows the use of more meaningful priors and smaller > box sizes.” > > Is this option (recentering and reboxing around the center of mass of a > subregion) available anywhere else in Relion 3, as an independent job > outside of the multi-body module? It would be a very handy option for > existing focused refinements, or during signal subtraction. > > Cheers > Oli > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > > ------------------------------ > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > -- “I’ve learned to think like a virus; no neurons, a lot of sex, and a lot of errors.” John J. Holland Biól. Sergio Aguirre Sampieri ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1