Hi Thomas and Joe,
    2D classification works after splitting the data into two halves. Hopefully this issue will be resolved in future releases.
  Best

> On Oct 26, 2018, at 5:14 AM, Joseph Atherton <[log in to unmask]> wrote:
>
> Yes, splitting the data in half worked nicely for me too when I had this error.
>
> Cheers,
> Joe
>
>> On 25/10/2018, 20:27, Yu Xinchao wrote:
>> Thanks Thomas, I do have a very large data set (9000+ movies) and that might be the reason. I will try split the data in half and rerun it.
>> Best
>> Xinchao
>>
>> Sent from my iPhone
>>
>>> On Oct 25, 2018, at 12:22 PM, Thomas Bausewein <[log in to unmask]> wrote:
>>>
>>> Hi Xinchao,
>>>
>>> im my case I had a really large dataset and the set2Dslice error vanished when I split the dataset in half (using the Relion GUI) and classified the smaller datasets individually.
>>> I did not remove any particles, neither from the folders nor from the particle.star files.
>>>
>>> You should also do relion_image_handler --stats --i Extract/Job006/particles.star in your project folder and not in the Extract/job006 folder, since it reads the path that is written in the particles.star file.
>>>
>>> Thomas
>>>
>>>
>>>> On 25 Oct 2018, at 20:59, Xinchao Yu <[log in to unmask]> wrote:
>>>>
>>>> Hi All, I got the the same error message when I tried to do 2D classfication: Backprojector::set2DSlice%%: Dimension of the data array should be 2 or 3
>>>> I was able do MotionCor2, Gctf, Autopicking and particle extraction without any error messages.
>>>> I checked the files with relion_image_handler --stats --i particles.star and it's always the first file that give the following message:
>>>> ERROR:
>>>>  Cannot read file Extract/Job006/Micrographs/NP146-g1_06973.mrcs It does not exist.
>>>> I checked the folder and the file does exist and when I deleted the file from the .star files I would get same error message for the next file.
>>>> Does anyone have a solution to this?
>>>> Thanks,
>>>> Xinchao Yu
>>>>
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