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Dear Elinor I was also looking for this information a while ago. The highest high resolution paper was with 2% DMSO of filament (microtubulin I think) and for a membrane protein was 0.1%. I am trying now, 2% and 4% which reduces the contrast of the image. however, as long as you can see the particle I guess that it is OK. Cheers Amir Dr. Amir Apelbaum, Ph.D. Department of Structural Biochemistry Max Planck Institute of Molecular Physiology Otto-Hahn-Str. 11 D-44227 Dortmund Tel: +49 (231) 133-2311 Fax: +49 (231) 133-2398 http://raunser-lab.de On 24. Oct 2018, at 09:31, Elinor Breiner <[log in to unmask]<mailto:[log in to unmask]>> wrote: Hi all What is the max conc. of DMSO you used on a grid? Do you have any recommendation for other organic solvents that can be used for single particle cryo EM? If so – what concentration did you use? Thanks in advance Elinor ________________________________ Elinor Breiner Goldstein, Ph.D. Student Department of Structural Biology @The Ribosome Group Room 607, The Kimmelman building Weizmann Institute of Science Herzl st. 234, Rehovot, 7610001 Israel Tel: 972-8-9343022 Fax:972-8-9344154 e-mail: [log in to unmask]<mailto:[log in to unmask]> ________________________________ To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1