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Dear Khursheed, As Richard said, Ximdisp is part of the MRC image processing suite, which you can download from the CCP-EM website and build for yourself. However, the MRC programs are also included in the CCP-EM package, so if you have CCP-EM 1.1 installed and set up you should be able to start Ximdisp by simply typing 'Ximdisp' on the command line. Thank you for pointing out the error when you try to use CCP-EM's image viewer to open a large micrograph. We will look into this and fix the problem. I should point out that the CCP-EM image viewer is useful for quickly checking images, but it doesn't include any particle marking or picking functions so you will need to use Ximdisp or other tools for looking at particle coordinates. Best wishes Colin -- Dr. Colin Palmer CCP-EM | www.ccpem.ac.uk | @ccp_em Research Complex at Harwell STFC Rutherford Appleton Laboratory Didcot OX11 0FA, UK +44 1235 567864 -----Original Message----- From: Collaborative Computational Project in Electron cryo-Microscopy <[log in to unmask]> On Behalf Of [log in to unmask] Sent: 21 October 2018 23:32 To: [log in to unmask] Subject: Re: [ccpem] Query related to Ximdis and CCP-EM - Need Help Dear Khursheed, To download and install Ximdisp, go to http://www.ccpem.ac.uk/download.php, then download image2010.tar.gz. Ximdisp is one of the programs in the suite of (older) MRC programs, written by Jude Short. The description on the web site follows. MRC Image Processing System - Tony Crowther, Richard Henderson & Jude Short (Smith) Installation is described in /image2010/README. First build all the libraries and executables using a .bld file specific to your system. Binaries will then be written to /image2010/bin and all have the extension .exe Richard > Hi, > > I'm doing my master's from "Indian Institute of Technology Bombay (IIT > Bombay), India" university in the field of Cryo-EM. Objective of my > work is to develop robust algorithm for "Particle picking" & "3D > tomographic reconstruction" in Cryo-EM. We have few hundred > micrographs (.mrc > format) and their particles markings taken from some external Biology LAB. > > Problem which we are facing is that, Biology LAB people had marked > particles on micrograph (.mrc format) done using "*Ximdis*" software. > They are saying that these micrograph and their particle marking can > be opened using Ximdis or CCP-EM tool. As we are new to CCP-EM tool, > we are not able to open these micrographs and their particle marking. > Also CCP-EM is crashing or sometime gives error, when micrograph of > very big dimension i.e 7000x7000 is opened. Below are the few > questions for which we are searching for the answers. > > 1. From where we can get XimDis tool? Because we are not able find > Ximdis. If there is any download site or link then please let us know. > > 2. How to open micrograph and their respective marking file (marking > is done using Ximdis)? > > 3. Also, if we just open big micrograph (7000x7000) from "image" > section (bottom-right menu) of CCP-EM 1.1 then it gives error. > > > Thanks and Regards, > > Khursheed Ali, > > M.Tech. - Computer Science and Engineering, > > Indian Institute of Technology Bombay (IIT Bombay), > > Mumbai, India > > ###################################################################### > ## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1