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Hello everyone- I am processing a dataset with Relion 3, and I realized that I have made a careless error. My data were collected in superresolution mode on a K2, but during motion correction I accidentally entered the non-superresolution, binned pixel size (e.g. 1.0 instead of 0.5). I’ve now gotten to the point where I would like to do Bayesian polishing, but I would prefer not to have to re-align all of the movies. Instead, it would be great if I could edit the star files which contain the trajectories so that they match the proper pixel size. So my question is, what are the units of the “_rlnMicrographShiftX” and “_rlnMicrographShiftY”? Are they in pixels, or Angstroms? I am hoping that simply editing these if they are in Angstroms, as well as the “_rlnMicrographOriginalPixelSize” will be sufficient. If anyone else has dealt with this little mistake successfully, any comments would be appreciated : ) Thanks, -Greg Alushin ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1