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Hi Xinchao,

Did your setting include clip to common subarea or drift correction when you took images?
I’ve had an issue caused by this setting. If yours is the similar case, I have corrected mine by doing the new_box in Relion (suggested by Sjors and it’s in the email thread of CCPEM (July 18th).

Best wishes,
Jeongyoon

----------------------
Jeongyoon Choi

Postdoctoral research fellow
Dept of Biochemistry
University of Oxford
OX1 3QU

Phone: +44 (0)1865 613228






On 30 Oct 2018, at 13:28, Yu Xinchao <[log in to unmask]> wrote:

Hi Sergey, 
  My data came from the same microscope, I will give it a try.
Xinchao

On Oct 30, 2018, at 6:25 AM, Sergey Nazarov <sergey.nazarov@UNIBAS
.CH> wrote:



---------- Forwarded message ---------
From: Sergey Nazarov <[log in to unmask]>
Date: вт, 30 окт. 2018 г. в 14:18
Subject: Re: [ccpem] Merge two data sets with different pixel sizes
To: <[log in to unmask]>


Hi Xinchao,

I did rescale-and-join approach, it might work if images are from the same microscope.

Best,
Sergey

вт, 30 окт. 2018 г. в 14:01, Yu Xinchao <[log in to unmask]>:
Hi Sergry, 
  That’s useful to read through, I was thinking maybe one could just rescale the particles during extraction and then join the star files using relion3. Has anybody tried this approach? Thanks 
 Xinchao 


On Oct 30, 2018, at 12:47 AM, Sergey Nazarov <[log in to unmask]> wrote:

Hi Xinchao,

Have a look at this thread

and attached file (unfortunately, don't remember the author).

Best,
Sergey Nazarov
CIME, EPFL, Lausanne

вт, 30 окт. 2018 г. в 5:42, Xinchao <[log in to unmask]>:
Hi all,
    I have two data sets of the same sample that were collected at slightly different pixel sizes (~5% difference), does anybody have a good way of merging the two data sets?
    Thanks,
     Xinchao

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