 |
 |
Dear Szymon,
these stripes also appear when there is an uneven angular assignment. This can be by the data itself (preferential interaction with the water-air interface, or the support), or by a problem of the angular assignment algorithm. You may check the angular distribution, ideally it should be even. If not, with side views alone you can reconstruct without any missing region in Fourier space. To know if it is a problem of the data or the algorithm, you would need to perform a second reconstruction with some other software.
Kind regards, Carlos Oscar
[log in to unmask]">Dear Takanori and Sjors
I have the same issue apparent only in unfiltered half maps (stable Relion 2.1):
I've been wondering what could be the origin of these artifactual streaks of density (attached).
With thanks for any possible advice,Szymon
Hi,
I was wondering if anyone managed to find a solution for this bug with unfiltered helical reconstruction during 3Drefine?
Things I have discovered since the original problem:
1) I get the same problem with relion v3.0
2) I don't get the same problem when I apply helical symmetry
Any help would be greatly appreciated,
Cheers,
Joe
########################################################################
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
-- ------------------------------------------------------------------------ Carlos Oscar Sánchez Sorzano e-mail: [log in to unmask] Biocomputing unit http://i2pc.es/coss National Center of Biotechnology (CSIC) c/Darwin, 3 Campus Universidad Autónoma (Cantoblanco) Tlf: 34-91-585 4510 28049 MADRID (SPAIN) Fax: 34-91-585 4506 ------------------------------------------------------------------------
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
