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Hi

Do you mean ximdisp ? The MRC format is pretty standard and should open in
most EM packages (IMOD ,EMAN2 etc) .



1.What format are your co-ordinates in ? (.box ,.star,spider) ?

Sincerely
Joshua

On Sun, 21 Oct 2018, 22:11 Khursheed Ali, <[log in to unmask]> wrote:

> Hi,
>
> I'm doing my master's from "Indian Institute of Technology Bombay (IIT
> Bombay), India" university in the field of Cryo-EM. Objective of my work is
> to develop robust algorithm for "Particle picking" & "3D tomographic
> reconstruction" in Cryo-EM. We have few hundred micrographs (.mrc format)
> and their particles markings taken from some external Biology LAB.
>
> Problem which we are facing is that, Biology LAB people had marked
> particles on micrograph (.mrc format) done using "*Ximdis*" software.
> They are saying that these micrograph and their particle marking can be
> opened using Ximdis or CCP-EM tool.  As we are new to CCP-EM tool, we are
> not able to open these micrographs  and their particle marking. Also CCP-EM
> is crashing or sometime gives error, when micrograph of very big dimension
> i.e 7000x7000 is opened.  Below are the few questions for which we are
> searching for the answers.
>
> 1. From where we can get XimDis tool? Because we are not able find Ximdis.
> If there is any download site or link  then please let us know.
>
> 2. How to open micrograph and their respective marking file (marking is
> done using Ximdis)?
>
> 3. Also, if we just open big micrograph (7000x7000) from "image" section
> (bottom-right menu) of  CCP-EM 1.1 then it gives error.
>
>
> Thanks and Regards,
>
> Khursheed Ali,
> M.Tech. - Computer Science and Engineering,
>
> Indian Institute of Technology Bombay (IIT Bombay),
> Mumbai, India
>
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