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Hi Dr Heumann You can remove the micrographs directly from the GUI without needing sed. Those warnings harmless AFAIK . Grouping by defocus is possibly more helpful than per micrograph Sincerely. Joshua On Fri, 12 Oct 2018, 21:11 John Heumann, <[log in to unmask]> wrote: > We have a dataset in which most micrographs contain 200 - 300 particles, > but a minority contain few or even no particles after 3D classification and > selection. Rather than regrouping all the data, it's been my practice to > gather the list of offending micrographs during an initial run of > re-extraction with refined coordinates, then to delete the corresponding > entries from the selected particles.star file with sed, and finally to run > a fresh extraction with the reduced set of refined coordinates. Is this > reasonable? When I do this, the number of extracted particles is reduced as > I'd expect, but for some reason there are still warnings during extraction > about micrographs with too few particles. Are these ignorable? > > On a related note, I see from the Subsets tab in Subset Selection that > there's new (or possibly in progress) work to facilitate screen based on > various criteria. Would it be possible to include min / max number of > particles per micrograph to such screens in the future? > > Thanks! > -jh- > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1