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Dear all cryo-EM experts, Jumped on the cryo-EM bandwagon recently to determine 3D structures of protein complexes. Does anyone have an experience how to mitigate a problem that a protein sample sticks to the carbon matrix of holey grids instead of being suspended in the vitreous ice spanned by the holes? Materials that I used are; Quantifoil R2/1 Cu 200 mesh and R1.2/1.3 Au 300 mesh A ~320 kDa protein complex supplemented with 20 mM Tris-HCl pH 7.5 and 200 mM NaCl Thanks in advance for sharing your experience. Kind regards, John ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1