I‘m not sure if I understand correctly, but would it be possible to apply symmetry expansion before the C1 refinement? That way you could get the full signal for your ASU without actually enforcing symmetry.Best,BasilIs there any documentation about using Relion 3 MultiBody Refinement for a particle with symmetry? Would applying symmetry violate the assumptions of the algorithm?--I've run multibody refine from a consensus refinement I did in C1 and treated the subunits as separate bodies. I clearly observe continuous structural heterogeneity, so is applying symmetry to these particles at the level of consensus refinement inappropriate?Cheers,Sara WeaverSara J. WeaverDoctoral Candidate, Jensen LabCalifornia Institute of Technology
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