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Dear Sara,

Our localized reconstruction method is suited well to tackle the problem you describe. It allows you to classify/refine the symmetry-mismatched/flexible subunits. You can use your Relion 3D refinement as a starting point. Please see Ilca et al 2015 Nat Commun and let me know if you need any help. 

Best wishes,
Juha

On Wed, Aug 22, 2018 at 10:42 PM Basil Greber <[log in to unmask]> wrote:
I‘m not sure if I understand correctly, but would it be possible to apply symmetry expansion before the C1 refinement? That way you could get the full signal for your ASU without actually enforcing symmetry.

Best,

Basil

Sara Weaver <[log in to unmask]> schrieb am Mi. 22. Aug. 2018 um 11:45:
Is there any documentation about using Relion 3 MultiBody Refinement for a particle with symmetry?  Would applying symmetry violate the assumptions of the algorithm?    

I've run multibody refine from a consensus refinement I did in C1 and treated the subunits as separate bodies.  I clearly observe continuous structural heterogeneity, so is applying symmetry to these particles at the level of consensus refinement inappropriate?  

Cheers,
Sara Weaver 
--
Sara J. Weaver
Doctoral Candidate, Jensen Lab
California Institute of Technology

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