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Dear Sara, Our localized reconstruction method is suited well to tackle the problem you describe. It allows you to classify/refine the symmetry-mismatched/flexible subunits. You can use your Relion 3D refinement as a starting point. Please see Ilca et al 2015 Nat Commun and let me know if you need any help. Best wishes, Juha On Wed, Aug 22, 2018 at 10:42 PM Basil Greber <[log in to unmask]> wrote: > I‘m not sure if I understand correctly, but would it be possible to apply > symmetry expansion before the C1 refinement? That way you could get the > full signal for your ASU without actually enforcing symmetry. > > Best, > > Basil > > Sara Weaver <[log in to unmask]> schrieb am Mi. 22. Aug. 2018 um 11:45: > >> Is there any documentation about using Relion 3 MultiBody Refinement for >> a particle with symmetry? Would applying symmetry violate the assumptions >> of the algorithm? >> >> I've run multibody refine from a consensus refinement I did in C1 and >> treated the subunits as separate bodies. I clearly observe continuous >> structural heterogeneity, so is applying symmetry to these particles at the >> level of consensus refinement inappropriate? >> >> Cheers, >> Sara Weaver >> -- >> Sara J. Weaver >> *Doctoral Candidate, Jensen Lab* >> *California Institute of Technology* >> >> ------------------------------ >> >> To unsubscribe from the CCPEM list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 >> > > ------------------------------ > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1 > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1