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I second (third?) what Tommi and Kevin said about using an oil to cover the drop to slow evaporation (I like paraffin for this—not too viscous). Here’s an additional nuance: Saturate the oil with the alcohol first, before using it to cover the drop. > On 14 Aug 2018, at 2:58 PM, Thomas Krey <[log in to unmask]> wrote: > > Dear crystallization experts, > > We have 3D protein crystals grown from a microseed matrix screening vapor diffusion experiment in either > > 15% (v/v) Reagent alcohol > HEPES Na pH 7.5 > 0.2 M MgCl2 > > or in > > 27% Isopropanol > 0.18 M MgCl2 > 90 mM HEPES Na pH 7.5 > 10% Glycerol > > Upon opening the corresponding wells these crystals move quite a bit – presumably due to the volatility of the alcohols. Does anyone have a good suggestion to stabilize the swirling movements? Does anyone have experience, whether these conditions alone can serve as cryo-protectant (i.e., do we really have to fish, move into cryo solution and fish again)? > Any suggestion or input would be highly welcome. > > Thank you very much in advance. > > Thomas > > > Prof. Dr. Thomas Krey > Hannover Medical School > Institute of Virology > Structural Virology Group > Carl-Neuberg-Str. 1 > D-30625 Hannover > phone: +49 (0) 511 - 532 4308 > email: [log in to unmask] > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102-1192 USA (215) 762-7706 [log in to unmask] [log in to unmask] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1