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Dear Careina, you could use the old crystals, that did not diffract, for microseeding to regrew nicer crystals. Once you have them, try to use them as quickly as possible. Three weeks can be a long time for crystals. Storage in liquid nitrogen should not be the problem. Best, Tim On 08/14/2018 11:58 AM, Careina Edgooms wrote: > I got the most beautiful crystals I have ever seen and they don't > diffract at all. Not poor diffraction, NO diffraction. Anyone know why > this could be and how I can go about fixing it? I had three beautiful > crystals and not one diffracted. I did leave them in the drop for about > 3 weeks before harvesting and in liquid nitrogen for about a month > before diffracting. Could that be a factor? If I regrew more beautiful > crystals and diffracted straight away could that help? > Careina > > ------------------------------------------------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- -- Paul Scherrer Institut Tim Gruene - persoenlich - OSUA/204 CH-5232 Villigen PSI phone: +41 (0)56 310 5297 GPG Key ID = A46BEE1A ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1