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I wouldnt have thought so.
There is a B factor plot as part of CCP4I2 - does that show any pattern of differences between ligand and environ,ment?

Eleanor

On 9 August 2018 at 10:54, Santhosh Gatreddy <[log in to unmask]> wrote:
Hi all,

I have to compare the B-factors of three of my ligand bound structures of the same protein which were diffracted to 1.85, 1.98 and 2.01 A resolution.

Is it necessary to normalize the B-factors of these structures (dimer in ASU) before comparing them to understand the ligand induced stability.


Thanks for Your suggestions,

Santhosh









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