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in addition to Rob and Israel's excellent suggestions you may also want to have a look at Garib's account in Chapter 11 here:

Best wishes,

Arjen

On Friday, 31 August 2018, F.Xavier Gomis-Rüth <[log in to unmask]> wrote:

Dear all,

is there some kind of general trend if not consensus as how to refine cryo-EM structures? What do people do for maps in the 3-4Å range,

pure RSR against the untouched map and that's it? Reciprocal space refinement against the backtransformed structure factors from the map

but without touching the map?

I've even heard that some people "dare" to calculate 2mFo-DFc-maps....

Thanks a lot for your feedback.

Best,

Xavier

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