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Dear crystallization experts, We have 3D protein crystals grown from a microseed matrix screening vapor diffusion experiment in either 15% (v/v) Reagent alcohol HEPES Na pH 7.5 0.2 M MgCl2 or in 27% Isopropanol 0.18 M MgCl2 90 mM HEPES Na pH 7.5 10% Glycerol Upon opening the corresponding wells these crystals move quite a bit – presumably due to the volatility of the alcohols. Does anyone have a good suggestion to stabilize the swirling movements? Does anyone have experience, whether these conditions alone can serve as cryo-protectant (i.e., do we really have to fish, move into cryo solution and fish again)? Any suggestion or input would be highly welcome. Thank you very much in advance. Thomas Prof. Dr. Thomas Krey Hannover Medical School Institute of Virology Structural Virology Group Carl-Neuberg-Str. 1 D-30625 Hannover phone: +49 (0) 511 - 532 4308 email: [log in to unmask] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1