 |
 |
Hello Zhou, How sure are you about the space group of the solution? Any signs of twinning or t-NCS? Best, Sudipta. On Mon, Aug 20, 2018, 7:36 PM SUBSCRIBE CCP4BB Zhu Qiao < [log in to unmask]> wrote: > Hi All > > My protein is dimer both in protein buffer and crystallisation reservoir, > which is confirmed by calibration column Supderdex 200 10/300 increase. > > The crystal can diffract to 3 and the space group was determined to be > P212121. > > The solvent content and Matthews coefficient shows > 1 copy, solvent content is 74.5% and Matthews coeff is 4.82 > 2 cpoies, solvent content is 49% and Matthews coeff is 2.41 . > > I did MR. I can only get one molecule in ASU and the R work / R free is > 0.31/0.35 after several cycles of refinement. There are some uncontinued > electron density, which indicating may be one more chain is there. > > I tried to search the other one by disallow the packing test or increase > the packing cut off value in phaser, I can get two molecules, but only one > has good fit to the electron density map. The other chain hardly have any > electron density. > > Does anyone have any experience regarding this situation? We appreciate > your help. > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1