Dear CAT12 expert, I am performing a VBM longitudinal studies on stroke patients and I´m having issues in the segmentation step in one of them. For preprocessing I have introduced the T1 image with the lesion areas set to zero (lesion masked). The error I find is " Error using cat_main_gintnorm (line 376)" I have also run the segmentation on the T1 image without taking out the lesion and runs well but I understand this will be less accurate due to tissue misclassification Any idea on how may I solve this? Thank you in advance Best Maria Jose Running 'Longitudinal Rigid Registration' Estimated noise sd for "F:\VBM_10patientsDone\P09\p09_t1\P09_T1_masked.img,1" = 9.04208 Estimated noise sd for "F:\VBM_10patientsDone\P09\p09_t2\P09_T1_masked.img,1" = 10.5562 Estimated noise sd for "F:\VBM_10patientsDone\P09\p09_t3\P09_T3_masked.img,1" = 8.92616 APP: Initialize 4s Estimate background 5s Initial correction 2s Refine background 6s Final correction 2s Final scaling 9s APP-noise ~ 16%, APP-bias ~ 57% (BWP-level), APP-inv ~ 0.00 55s Done 'Longitudinal Rigid Registration' Running 'CAT12: Segmentation' ------------------------------------------------------------------------ CAT12.3 r1318: 1/1: F:\VBM_10patientsDone\P09\p09_t1\avg_P09_T1_masked.n ------------------------------------------------------------------------ SANLM denoising (NCstr=-Inf): 38s APP: Rough bias correction: Initialize 10s Estimate background 9s Initial correction 14s Refine background 12s Final correction 11s Background correction 1s Final scaling 3s 60s Coarse affine registration 8s Affine registration 18s SPM preprocessing 1 (estimate): 446s SPM preprocessing 2 (write): 88s Global intensity correction: ------------------------------------------------------------------------ CAT Preprocessing error for avg_P09_T1_masked: ------------------------------------------------------------------------ Bad tissue contrast (C=373.01, G=690.27, W=-0.14) ------------------------------------------------------------------------ 376 - cat_main_gintnorm 526 - cat_main 674 - cat_run_job1070 16 - cat_run_newcatch 392 - run_job 161 - cat_run 29 - cfg_run_cm 1688 - local_runcj 959 - cfg_util 469 - fill_run_job 247 - spm_jobman 53 - cat_long_multi_run 29 - cfg_run_cm 1688 - local_runcj 959 - cfg_util 703 - MenuFileRun_Callback 95 - gui_mainfcn 53 - cfg_ui ------------------------------------------------------------------------ Warning:cat_io_report:CATgui: Incomplete report creation in cat_io_report because of incomplete CAT parameters. Print 'Graphics' figure to: F:\VBM_10patientsDone\P09\p09_t1\report\catreport_avg_P09_T1_masked.pdf Failed 'CAT12: Segmentation' Error using cat_main_gintnorm (line 376) Bad tissue contrast (C=373.01, G=690.27, W=-0.14)