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Dear all, I have picked my helix in eman2 using e2helixboxer.py and I loaded the coordinates into RELION. But I noticed there is a shift in the helix I picked. Also, the micrographs shown in e2helixboxer.py viewer is upside-down to the images displayed in RELION. I am wondering what's going on? Best, Yangqi ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1